הרצאת אורח המחלקה להנדסה ביו רפואית

Sperm Morphological Analysis with Interferometric Phase Microscopy

Dr. Michal Balberg

Visiting Scholar, Dept. of Biomedical Engineering, Tel Aviv University

Senior Lecturer, Holon Institute of Technology, Israel

Sperm cell morphology is analyzed for assessing the fertility potential of a male. Currently, it is performed by staining the cells with a dye that creates a contrast between morphological features, such as the nucleus and the acrosome, and imaging it with a bright field microscope. Interferometric phase microscopy (IPM) is an imaging technique capable of capturing both amplitude and phase maps of transparent biological cells, and extracting the optical thickness of the cell. We show that according to the WHO criteria for morphological analysis, IPM provides equivalent information to the current practice. Following, we show that we can extract, without staining, other physiological parameters, such as the dry mass of the cell. These initial findings may assist in selecting unstained sperm cells for in-vitro cytoplasmic injection (ICSI) in the future, based on their morphological features, where staining is not allowed.

ההרצאה תתקיים ביום ראשון 27.11.16, בשעה 15:00

בחדר 315, הבניין הרב תחומי, אוניברסיטת תל אביב